SARS-CoV-2 specific neutralising antibodies in blood donors

We evaluated SARS-CoV-2 RNA and neutralising antibodies in blood donors (BD) residing within the Lodi Purple Zone, Italy. Of 390 BDs recruited after 20 February 2020 – when the primary COVID-19 case in Lombardy was recognized, 91 (23%) aged 19-70 years had been antibody constructive.

                     
                     

Viral RNA was detected in an extra 17 (4.3%) BDs, yielding ca 28% (108/390) with proof of virus publicity. 5 saved samples collected as early as 12 February had been seropositive.

                     
                     

Key phrases: Lodi Purple Zone; SARS-CoV-2 seroprevalence; epidemiology; microneutralization assay.

                     
                     

                                           

NMDAR Antibodies Alter Dopamine Receptors and Trigger Psychotic Conduct in Mice

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Goal: The goal was to exhibit that antibodies from sufferers with anti-N-methyl-d-aspartate receptor (NMDAR) encephalitis alter the degrees of dopamine 1 receptor (D1R) and dopamine 2 receptor (D2R) and trigger psychotic-like options in mice.

                     
                     

T-Cell Immunoglobulin Mucin Receptor 3 (TIM-3) Antibody (FITC)

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T-Cell Immunoglobulin Mucin Receptor 3 (TIM-3) Antibody (RPE)

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Human T-Cell Immunoglobulin Mucin Receptor 3 (TIM-3) Antibody (Biotin Conjugate)

32146-05121 150 ug
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T-Cell Immunoglobulin Mucin Receptor 1 (TIM-1) Antibody

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T-Cell Immunoglobulin Mucin Receptor 1 (TIM-1) Antibody

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T-Cell Immunoglobulin Mucin Receptor 4 (TIM-4) Antibody

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T-Cell Immunoglobulin Mucin Receptor 4 (TIM-4) Antibody

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T-Cell Immunoglobulin Mucin Receptor 1 (TIM-1) Antibody

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T-Cell Immunoglobulin Mucin Receptor 1 (TIM-1) Antibody

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T-Cell Immunoglobulin Mucin Receptor 4 (TIM-4) Antibody

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Recombinant Mouse T cell Immunoglobulin and Mucin Domain-3/TIM-3/HAVCR2 (C-6His)

CK57-10ug 10ug
EUR 132
Description: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.

Recombinant Mouse T cell Immunoglobulin and Mucin Domain-3/TIM-3/HAVCR2 (C-6His)

CK57-1mg 1mg
EUR 2008.8
Description: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.

Recombinant Mouse T cell Immunoglobulin and Mucin Domain-3/TIM-3/HAVCR2 (C-6His)

CK57-500ug 500ug
EUR 1423.2
Description: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.

Recombinant Mouse T cell Immunoglobulin and Mucin Domain-3/TIM-3/HAVCR2 (C-6His)

CK57-50ug 50ug
EUR 278.4
Description: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.

Recombinant Mouse T Cell Immunoglobulin and Mucin Domain-3/TIM-3/HAVCR2 (C-Fc)

CM03-10ug 10ug
EUR 151.2
Description: Lyophilized from a 0.2 μm filtered solution of PBS,pH7.4.

Recombinant Mouse T Cell Immunoglobulin and Mucin Domain-3/TIM-3/HAVCR2 (C-Fc)

CM03-1mg 1mg
EUR 2252.4
Description: Lyophilized from a 0.2 μm filtered solution of PBS,pH7.4.

Recombinant Mouse T Cell Immunoglobulin and Mucin Domain-3/TIM-3/HAVCR2 (C-Fc)

CM03-500ug 500ug
EUR 1593.6
Description: Lyophilized from a 0.2 μm filtered solution of PBS,pH7.4.

Recombinant Mouse T Cell Immunoglobulin and Mucin Domain-3/TIM-3/HAVCR2 (C-Fc)

CM03-50ug 50ug
EUR 278.4
Description: Lyophilized from a 0.2 μm filtered solution of PBS,pH7.4.

Recombinant Mouse T Cell Immunoglobulin and Mucin Domain-3/TIM-3/HAVCR2 (C-6His)

CM54-10ug 10ug
EUR 151.2
Description: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.

Recombinant Mouse T Cell Immunoglobulin and Mucin Domain-3/TIM-3/HAVCR2 (C-6His)

CM54-1mg 1mg
EUR 2252.4
Description: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.

Recombinant Mouse T Cell Immunoglobulin and Mucin Domain-3/TIM-3/HAVCR2 (C-6His)

CM54-500ug 500ug
EUR 1593.6
Description: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.

Recombinant Mouse T Cell Immunoglobulin and Mucin Domain-3/TIM-3/HAVCR2 (C-6His)

CM54-50ug 50ug
EUR 278.4
Description: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.

Human T-Cell Immunoglobulin Mucin Receptor 3 (TIM-3) AssayLite Antibody (FITC Conjugate)

32146-05141 150 ug
EUR 513.6

Human T-Cell Immunoglobulin Mucin Receptor 3 (TIM-3) AssayLite Antibody (RPE Conjugate)

32146-05151 150 ug
EUR 513.6

Human T-Cell Immunoglobulin Mucin Receptor 3 (TIM-3) AssayLite Antibody (APC Conjugate)

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Human T-Cell Immunoglobulin Mucin Receptor 3 (TIM-3) AssayLite Antibody (PerCP Conjugate)

32146-05171 150 ug
EUR 565.2

T-Cell Immunoglobulin Mucin Receptor 1 (TIM-1) Antibody (FITC)

abx415205-01mg 0.1 mg
EUR 610.8

T-Cell Immunoglobulin Mucin Receptor 1 (TIM-1) Antibody (RPE)

abx415207-100tests 100 tests
EUR 710.4

Polyclonal Goat anti-GST p-form

GST-ANTI-3 50 uL
EUR 336

Recombinant Human T Cell Immunoglobulin and Mucin Domain-3/TIM-3/HAVCR2 (C-Fc-6His)

CX71-10ug 10ug
EUR 145.2
Description: Lyophilized from a 0.2 μm filtered solution of PBS,pH7.4.

Recombinant Human T Cell Immunoglobulin and Mucin Domain-3/TIM-3/HAVCR2 (C-Fc-6His)

CX71-1mg 1mg
EUR 2739.6
Description: Lyophilized from a 0.2 μm filtered solution of PBS,pH7.4.

Recombinant Human T Cell Immunoglobulin and Mucin Domain-3/TIM-3/HAVCR2 (C-Fc-6His)

CX71-500ug 500ug
EUR 1935.6
Description: Lyophilized from a 0.2 μm filtered solution of PBS,pH7.4.

Recombinant Human T Cell Immunoglobulin and Mucin Domain-3/TIM-3/HAVCR2 (C-Fc-6His)

CX71-50ug 50ug
EUR 315.6
Description: Lyophilized from a 0.2 μm filtered solution of PBS,pH7.4.

T-Cell Immunoglobulin Mucin Receptor 3 (TIM3) Antibody

20-abx225210
  • EUR 444.00
  • EUR 727.20
  • EUR 376.80
  • 100 ul
  • 200 ul
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T-Cell Immunoglobulin Mucin Receptor 3 (TIM3) Antibody

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EUR 460.8

Jurkat T / GFP Stable Cell (CD43 Promoter)

SC049-3 2 x 106 cell/ml x 1ml
EUR 1800
Description: GFP expression stable cell line in human Jurkat T Cells with Puromycin resistance. GFP is expressed under the promoter of human CD43 gene.

T-Cell Immunoglobulin And Mucin Domain-Containing Protein 2 (TIM-2) Antibody

abx415208-05mg 0.5 mg
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T-Cell Immunoglobulin And Mucin Domain-Containing Protein 2 (TIM-2) Antibody

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Anti-14-3-3 alpha + beta Rabbit Monoclonal Antibody

M02431-3 100ug/vial
EUR 476.4
Description: Rabbit Monoclonal 14-3-3 alpha + beta Antibody. Validated in Flow Cytometry, IP, IF, IHC, ICC, WB and tested in Human, Mouse, Rat.

Anti-VEGF Receptor 3/FLT4 Antibody

A01276-3 100ug/vial
EUR 400.8

T-Cell Immunoglobulin And Mucin Domain-Containing Protein 2 (TIM-2) Antibody (FITC)

abx415209-01mg 0.1 mg
EUR 610.8

Anti-active Caspase-3 Rabbit Monoclonal Antibody

M00334-3 100ug/vial
EUR 476.4
Description: Anti-active Caspase-3 Rabbit Monoclonal Antibody tested for IF, IHC, ICC, WB in Human

Anti-Galectin 3/LGALS3 Antibody (monoclonal, 12B12)

M00621-3 100ug/vial
EUR 400.8

Anti-Glyceraldehyde 3-Phosphate Dehydrogenase [GAPDH] Monoclonal Antibody

M00227-3 100ul
EUR 476.4
Description: Mouse Monoclonal Glyceraldehyde 3-Phosphate Dehydrogenase [GAPDH] Antibody. Validated in IHC, WB and tested in Equine.

MouseExoPure? 0.4 micron Immunobeads (Overall Exosome Isolation, mouse cell media, 3 reactions)

M1036-3
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MouseExoPure? 1.0 micron Immunobeads (Overall Exosome Isolation, mouse cell media, 3 reactions)

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ExoPure? 0.4 micron Immunobeads (Overall Exosome Isolation, cell media, 3 reactions)

M1030-3
EUR 529.2

ExoPure? 1.0 micron Immunobeads (Overall Exosome Isolation, cell media, 3 reactions)

M1031-3
EUR 523.2

IL-3 Interleukin-3 Human Recombinant Protein, His Tag

PROTP08700-3 Regular: 50ug
EUR 380.4
Description: Interleukin-3 Human Recombinant produced in E.Coli is single, a non-glycosylated, Polypeptide chain containing 154 amino acids fragment (20-152) and having a total molecular mass of 17.3kDa and fused with a 20 aa N-terminal His tag. ;The IL3 His is purified by proprietary chromatographic techniques.

Recombinant Human T-cell Immunoglobulin and Mucin Domain-containing Protein 4/Tim-4 (C-6His)

CU18-10ug 10ug
EUR 169.2
Description: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.

Recombinant Human T-cell Immunoglobulin and Mucin Domain-containing Protein 4/Tim-4 (C-6His)

CU18-1mg 1mg
EUR 2008.8
Description: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.

Recombinant Human T-cell Immunoglobulin and Mucin Domain-containing Protein 4/Tim-4 (C-6His)

CU18-500ug 500ug
EUR 1423.2
Description: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.

Recombinant Human T-cell Immunoglobulin and Mucin Domain-containing Protein 4/Tim-4 (C-6His)

CU18-50ug 50ug
EUR 363.6
Description: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.

Mouse Monoclonal anti-PEG (backbone specific) IgG (Clone #3)

PEG13-M-3 100 ug
EUR 578.4

Individual Reaction Mix 3

G065-3 200 reactions
EUR 200.4

3-D Life Thioglycerol

T10-3 180 µl
EUR 57.6

MouseExoPure? 0.4 micron Immunobeads (Overall Exosome Isolation, mouse biofluids, 3 reactions)

M1034-3
EUR 398.4

MouseExoPure? 1.0 micron Immunobeads (Overall Exosome Isolation, mouse biofluids, 3 reactions)

M1035-3
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Western Blot Box - 2 7/8 x 1 3/16 x 3/4in.; 7.3 x 3 x 1.9cm

B1200-3 5/pack
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Description: Western Blot Boxes

FSH (Human Follicle-stimulating hormone) ELISA test

3 96T/Box Ask for price
Description: ELISA based test for quantitative detection of FSH (Human Follicle-stimulating hormone)

TGF-b-3 Transforming Growth Factor-Beta 3 Human Recombinant Protein, Plant

PROTP10600-3 Regular: 5ug
EUR 380.4
Description: TGFB3 Human Recombinant produced in plant is a disulfide-linked homodimeric, glycosylated, polypeptide chain containing 118 amino acids and having a molecular mass of 27.2kDa. ;The TGFB3 is fused to 6xHis tag at N-terminus and purified by standard chromatographic techniques.

3-D-Life SG-PVA

M81-3 3x 170 µl
EUR 177.6

3-D Life FG-PVA

M82-3 3x 170 µl
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3-D Life SG-Dextran

M91-3 3x 170 µl
EUR 188.4

3-D Life FG-Dextran

M92-3 3x 170 µl
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3-D Life RGD Peptide

P10-3 3x 1 µmol
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3-D Life PEG-Link

L50-3 3x 200 µl
EUR 112.8

3-D Life CD-Link

L60-3 3 x 200 µl
EUR 385.2

Dr. P Kit-Solution 3

K2021010-3 50 ml
EUR 159.6
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Recombinant Human MMP-3 Protein

PROTP08254-3 10ug
EUR 380.4
Description: Matrix metalloproteinases (MMPs) are a family of endoproteases that require zinc and calcium for expressing catalytic activity. These enzymes play a central role in the maintenance and remodeling of the extracellular matrix. Elevated expression of their activity, caused either by up-regulation of their expression or down-regulation of their cognate inhibitors, has been implicated in various degenerative disorders, including arthritis, cardiovascular disease, skeletal growth-plate disorders, and cancer metastasis. MMP-3 degrades fibronectin, laminin, collagens III, IV, and X, and cartilage proteoglycans. Recombinant human MMP-3 is a 42.8 kDa protein containing the entire catalytic N-terminal domain and the C-terminal domain (378 amino acids).

                     

Strategies: Cultured rat hippocampal neurons had been handled with cerebrospinal fluid (CSF) from sufferers with anti-NMDAR encephalitis or controls, and the consequences on clusters of D1R and D2R had been quantified. In vivo research included 71 C57BL/6J mice that had been chronically infused with CSF from sufferers or controls by way of ventricular catheters linked to subcutaneous osmotic pumps.

                     

Prepulse inhibition of the acoustic startling reflex (PPI; a marker of psychotic-like habits), reminiscence, locomotor exercise, and the density of cell-surface and synaptic D1R, D2R, and NMDAR clusters had been examined at completely different time factors utilizing reported strategies.

                     

Outcomes: In cultured neurons, CSF from sufferers, however not from controls, brought about a major lower of cell-surface D1R and a rise of D2R clusters. In mice, CSF from sufferers brought about a major lower of synaptic and complete cell-surface D1R clusters and a rise of D2R clusters related to a lower of PPI.

                     

These results had been accompanied by reminiscence impairment and a discount of floor NMDARs, as reported on this mannequin. The psychotic-like options, reminiscence impairment, and modifications in ranges of D1R, D2R, and NMDAR progressively improved a number of days after the infusion of CSF from sufferers stopped.

                     

Interpretation: Along with reminiscence deficits and discount of NMDARs, CSF antibodies from sufferers with anti-NMDAR encephalitis trigger reversible psychotic-like options accompanied by modifications (D1R lower, D2R enhance) in cell-surface dopamine receptor clusters. ANN NEUROL 2020.

                     

Complement activation by human IgG antibodies to galactose-α-1,3-galactose

                       

Some human antibodies might paradoxically inhibit complement activation on micro organism and improve pathogen survival in people. This property was additionally claimed for IgG antibodies reacting with terminal galactose-α-1,3-galactose (Galα3Gal; IgG anti-αGal), a naturally occurring and considerable antibody in human plasma that targets quite a few completely different pathogens.                      

  • To reinvestigate these results, we used IgG anti-αGal affinity remoted from a pool of regular human IgG and human hypogammaglobulinaemia serum as a complement supply.
  •                

 

  • Move cytometry was carried out to look at antibody binding and complement deposition on pig erythrocytes, Escherichia coli O86 and Streptococcus pneumoniae serotype 9V.
  •                

 

  • Particular nanobodies had been used to dam the impact of single complement components and to delineate the complement pathways concerned.
  •                  

                     

IgG anti-αGal was able to activating the classical complement pathway on all of the examined goal cells. The diploma of activation was exponentially associated to the density of sure antibody on E. coli O86 and pig erythrocytes, however extra linearly on S. pneumoniae 9V.                      

 

Leukotriene B4

B7011-.025 25 µg
EUR 255.6

Leukotriene B4

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EUR 433.2

Leukotriene B4

B7011-.1 100 µg
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Human Total Leukotriene ELISA kit

E01L0023-192T 192 tests
EUR 1524
Description: A Sandwich ELISA for quantitative measurement of Human Total Leukotriene in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Total Leukotriene ELISA kit

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Description: A Sandwich ELISA for quantitative measurement of Human Total Leukotriene in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Total Leukotriene ELISA kit

E01L0023-96 1 plate of 96 wells
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Human Leukotriene A4 ELISA kit

E01L0035-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Human Leukotriene A4 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Leukotriene A4 ELISA kit

E01L0035-48 1 plate of 48 wells
EUR 624
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Human Leukotriene A4 ELISA kit

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Human Leukotriene C4 ELISA kit

E01L0036-192T 192 tests
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Description: A competitive ELISA for quantitative measurement of Human Leukotriene C4 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Leukotriene C4 ELISA kit

E01L0036-48 1 plate of 48 wells
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Description: A competitive ELISA for quantitative measurement of Human Leukotriene C4 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Leukotriene C4 ELISA kit

E01L0036-96 1 plate of 96 wells
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Description: A competitive ELISA for quantitative measurement of Human Leukotriene C4 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Leukotriene B4 ELISA kit

E01L0037-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Human Leukotriene B4 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Leukotriene B4 ELISA kit

E01L0037-48 1 plate of 48 wells
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Description: A competitive ELISA for quantitative measurement of Human Leukotriene B4 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Leukotriene B4 ELISA kit

E01L0037-96 1 plate of 96 wells
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Description: A competitive ELISA for quantitative measurement of Human Leukotriene B4 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Leukotriene D4 ELISA kit

E01L0287-192T 192 tests
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Human Leukotriene D4 ELISA kit

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Human Leukotriene D4 ELISA kit

E01L0287-96 1 plate of 96 wells
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Human Leukotriene E4 ELISA kit

E01L0289-192T 192 tests
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Human Leukotriene E4 ELISA kit

E01L0289-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Human Leukotriene E4 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Leukotriene E4 ELISA kit

E01L0289-96 1 plate of 96 wells
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Description: A competitive ELISA for quantitative measurement of Human Leukotriene E4 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human leukotriene E4 ELISA kit

55R-1976 96 tests
EUR 740.4
Description: ELISA Kit for detection of leukotriene E4 in the research laboratory

Human Leukotriene,LT ELISA Kit

201-12-1954 96 tests
EUR 528
Description: A quantitative ELISA kit for measuring Human in samples from biological fluids.

Human Leukotriene B4 ELISA Kit

ELA-E0562h 96 Tests
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Human Leukotriene C4 ELISA Kit

ELA-E0619h 96 Tests
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Human leukotriene D4 ELISA Kit

ELA-E0935h 96 Tests
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Human Leukotriene(LT)ELISA Kit

GA-E1970HM-48T 48T
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Human Leukotriene(LT)ELISA Kit

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Human Leukotriene(LT)ELISA Kit

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Human Leukotriene A4, LTA4 ELISA Kit

CELI-66028h 96 Tests
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Human Leukotriene D4, LTD4 ELISA Kit

CELI-66050h 96 Tests
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Human Leukotriene C4, LTC4 ELISA Kit

CELI-66083h 96 Tests
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Human Leukotriene B4, LTB4 ELISA Kit

CELI-66088h 96 Tests
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Human Leukotriene B4 (LTB4) CLIA Kit

abx197218-96tests 96 tests
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Human Leukotriene C4 (LTC4) CLIA Kit

abx197221-96tests 96 tests
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Human Leukotriene D4 (LTD4) CLIA Kit

abx197224-96tests 96 tests
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Human Leukotriene A4 Hydrolase (LTA4H) Protein

20-abx654185
  • EUR 811.20
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  • EUR 2498.40
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  • 100 ug
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  • 1 mg
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Human Leukotriene E4, LTE4 ELISA Kit

ELA-E0507h 96 Tests
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Cysteinyl Leukotriene ELISA kit

55R-IB09606 96 wells
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Leukotriene B4 ELISA Kit

E4793-100 96 assays
EUR 697.2

 

The choice pathway of complement amplified complement deposition. Deposited C3 fragments coated the activating IgG anti-αGal, obstructing its detection and highlighting this as a possible common caveat in research of antibody density and complement deposition.
                     

 

 

The inherent capability for complement activation by the purified carbohydrate reactive IgG anti-αGal was much like that of regular human IgG. We suggest that the beforehand reported complement inhibition by IgG anti-αGal pertains to suboptimal assay configurations, in distinction to the complement activating property of the antibodies demonstrated on this paper.