SARS-CoV-2 specific neutralising antibodies in blood donors

We evaluated SARS-CoV-2 RNA and neutralising antibodies in blood donors (BD) residing within the Lodi Purple Zone, Italy. Of 390 BDs recruited after 20 February 2020 – when the primary COVID-19 case in Lombardy was recognized, 91 (23%) aged 19-70 years had been antibody constructive.

                     
                     

Viral RNA was detected in an extra 17 (4.3%) BDs, yielding ca 28% (108/390) with proof of virus publicity. 5 saved samples collected as early as 12 February had been seropositive.

                     
                     

Key phrases: Lodi Purple Zone; SARS-CoV-2 seroprevalence; epidemiology; microneutralization assay.

                     
                     

                                           

NMDAR Antibodies Alter Dopamine Receptors and Trigger Psychotic Conduct in Mice

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Goal: The goal was to exhibit that antibodies from sufferers with anti-N-methyl-d-aspartate receptor (NMDAR) encephalitis alter the degrees of dopamine 1 receptor (D1R) and dopamine 2 receptor (D2R) and trigger psychotic-like options in mice.

                     
                     

T-Cell Immunoglobulin Mucin Receptor 3 (TIM-3) Antibody
abx415213-01mg 0.1 mg
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Human T-Cell Immunoglobulin Mucin Receptor 3 (TIM-3) Antibody
32146-05111 150 ug
EUR 261
T-Cell Immunoglobulin Mucin Receptor 3 (TIM-3) Antibody (FITC)
abx415212-01mg 0.1 mg
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T-Cell Immunoglobulin Mucin Receptor 3 (TIM-3) Antibody (RPE)
abx415214-100tests 100 tests
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Human T-Cell Immunoglobulin Mucin Receptor 3 (TIM-3) Antibody (Biotin Conjugate)
32146-05121 150 ug
EUR 369
Recombinant Mouse T cell Immunoglobulin and Mucin Domain-3/TIM-3/HAVCR2 (C-6His)
CK57-10ug 10ug
EUR 110
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Recombinant Mouse T cell Immunoglobulin and Mucin Domain-3/TIM-3/HAVCR2 (C-6His)
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EUR 1674
Description: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.
Recombinant Mouse T cell Immunoglobulin and Mucin Domain-3/TIM-3/HAVCR2 (C-6His)
CK57-500ug 500ug
EUR 1186
Description: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.
Recombinant Mouse T cell Immunoglobulin and Mucin Domain-3/TIM-3/HAVCR2 (C-6His)
CK57-50ug 50ug
EUR 232
Description: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.
Recombinant Mouse T Cell Immunoglobulin and Mucin Domain-3/TIM-3/HAVCR2 (C-Fc)
CM03-10ug 10ug
EUR 126
Description: Lyophilized from a 0.2 μm filtered solution of PBS,pH7.4.
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CM03-50ug 50ug
EUR 232
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Recombinant Mouse T Cell Immunoglobulin and Mucin Domain-3/TIM-3/HAVCR2 (C-6His)
CM54-10ug 10ug
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Description: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.
Recombinant Mouse T Cell Immunoglobulin and Mucin Domain-3/TIM-3/HAVCR2 (C-6His)
CM54-1mg 1mg
EUR 1877
Description: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.
Recombinant Mouse T Cell Immunoglobulin and Mucin Domain-3/TIM-3/HAVCR2 (C-6His)
CM54-500ug 500ug
EUR 1328
Description: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.
Recombinant Mouse T Cell Immunoglobulin and Mucin Domain-3/TIM-3/HAVCR2 (C-6His)
CM54-50ug 50ug
EUR 232
Description: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.
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abx411874-01mg 0.1 mg
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32146-05141 150 ug
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Human T-Cell Immunoglobulin Mucin Receptor 3 (TIM-3) AssayLite Antibody (RPE Conjugate)
32146-05151 150 ug
EUR 428
Human T-Cell Immunoglobulin Mucin Receptor 3 (TIM-3) AssayLite Antibody (APC Conjugate)
32146-05161 150 ug
EUR 428
Human T-Cell Immunoglobulin Mucin Receptor 3 (TIM-3) AssayLite Antibody (PerCP Conjugate)
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T-Cell Immunoglobulin Mucin Receptor 1 (TIM-1) Antibody (FITC)
abx415205-01mg 0.1 mg
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T-Cell Immunoglobulin Mucin Receptor 1 (TIM-1) Antibody (RPE)
abx415207-100tests 100 tests
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Recombinant Human T Cell Immunoglobulin and Mucin Domain-3/TIM-3/HAVCR2 (C-Fc-6His)
CX71-10ug 10ug
EUR 121
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Recombinant Human T Cell Immunoglobulin and Mucin Domain-3/TIM-3/HAVCR2 (C-Fc-6His)
CX71-1mg 1mg
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Recombinant Human T Cell Immunoglobulin and Mucin Domain-3/TIM-3/HAVCR2 (C-Fc-6His)
CX71-500ug 500ug
EUR 1613
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Recombinant Human T Cell Immunoglobulin and Mucin Domain-3/TIM-3/HAVCR2 (C-Fc-6His)
CX71-50ug 50ug
EUR 263
Description: Lyophilized from a 0.2 μm filtered solution of PBS,pH7.4.
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Jurkat T / GFP Stable Cell (CD43 Promoter)
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EUR 397
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CU18-10ug 10ug
EUR 141
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IL-3 Interleukin-3 Human Recombinant Protein, His Tag
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PEG13-M-3 100 ug
EUR 482
Individual Reaction Mix 3
G065-3 200 reactions
EUR 167
3-D Life Thioglycerol
T10-3 180 µl
EUR 48
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B1200-3 5/pack
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M1034-3
EUR 332
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M1035-3
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TGF-b-3 Transforming Growth Factor-Beta 3 Human Recombinant Protein, Plant
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K2021010-3 50 ml
EUR 133
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L50-3 3x 200 µl
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M81-3 3x 170 µl
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M91-3 3x 170 µl
EUR 157
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M92-3 3x 170 µl
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3-D Life RGD Peptide
P10-3 3x 1 µmol
EUR 276

                     

Strategies: Cultured rat hippocampal neurons had been handled with cerebrospinal fluid (CSF) from sufferers with anti-NMDAR encephalitis or controls, and the consequences on clusters of D1R and D2R had been quantified. In vivo research included 71 C57BL/6J mice that had been chronically infused with CSF from sufferers or controls by way of ventricular catheters linked to subcutaneous osmotic pumps.

                     

Prepulse inhibition of the acoustic startling reflex (PPI; a marker of psychotic-like habits), reminiscence, locomotor exercise, and the density of cell-surface and synaptic D1R, D2R, and NMDAR clusters had been examined at completely different time factors utilizing reported strategies.

                     

Outcomes: In cultured neurons, CSF from sufferers, however not from controls, brought about a major lower of cell-surface D1R and a rise of D2R clusters. In mice, CSF from sufferers brought about a major lower of synaptic and complete cell-surface D1R clusters and a rise of D2R clusters related to a lower of PPI.

                     

These results had been accompanied by reminiscence impairment and a discount of floor NMDARs, as reported on this mannequin. The psychotic-like options, reminiscence impairment, and modifications in ranges of D1R, D2R, and NMDAR progressively improved a number of days after the infusion of CSF from sufferers stopped.

                     

Interpretation: Along with reminiscence deficits and discount of NMDARs, CSF antibodies from sufferers with anti-NMDAR encephalitis trigger reversible psychotic-like options accompanied by modifications (D1R lower, D2R enhance) in cell-surface dopamine receptor clusters. ANN NEUROL 2020.

                     

Complement activation by human IgG antibodies to galactose-α-1,3-galactose

                       

Some human antibodies might paradoxically inhibit complement activation on micro organism and improve pathogen survival in people. This property was additionally claimed for IgG antibodies reacting with terminal galactose-α-1,3-galactose (Galα3Gal; IgG anti-αGal), a naturally occurring and considerable antibody in human plasma that targets quite a few completely different pathogens.                      

  • To reinvestigate these results, we used IgG anti-αGal affinity remoted from a pool of regular human IgG and human hypogammaglobulinaemia serum as a complement supply.
  •                

 

  • Move cytometry was carried out to look at antibody binding and complement deposition on pig erythrocytes, Escherichia coli O86 and Streptococcus pneumoniae serotype 9V.
  •                

 

  • Particular nanobodies had been used to dam the impact of single complement components and to delineate the complement pathways concerned.
  •                  

                     

IgG anti-αGal was able to activating the classical complement pathway on all of the examined goal cells. The diploma of activation was exponentially associated to the density of sure antibody on E. coli O86 and pig erythrocytes, however extra linearly on S. pneumoniae 9V.                      

 

Polyclonal Goat anti-GST μ-form

GST-ANTI-2 50 uL
EUR 280

Polyclonal Goat anti-GST p-form

GST-ANTI-3 50 uL
EUR 280

Leukotriene B4

B7011-.025 25 µg
EUR 213

Leukotriene B4

B7011-.05 50 µg
EUR 361

Leukotriene B4

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EUR 641

Human Leukotriene,LT ELISA Kit

201-12-1954 96 tests
EUR 440
  • This Leukotriene ELISA kit is validated to work with samples from whole blood, serum, plasma and cell culture supernatant.
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Human leukotriene E4 ELISA kit

55R-1976 96 tests
EUR 617
Description: ELISA Kit for detection of leukotriene E4 in the research laboratory

Human Total Leukotriene ELISA kit

E01L0023-192T 192 tests
EUR 1270
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
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Description: A Sandwich ELISA for quantitative measurement of Human Total Leukotriene in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Total Leukotriene ELISA kit

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Description: A Sandwich ELISA for quantitative measurement of Human Total Leukotriene in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Total Leukotriene ELISA kit

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EUR 685
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
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Description: A Sandwich ELISA for quantitative measurement of Human Total Leukotriene in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Leukotriene A4 ELISA kit

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Description: A competitive ELISA for quantitative measurement of Human Leukotriene A4 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Leukotriene A4 ELISA kit

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Description: A competitive ELISA for quantitative measurement of Human Leukotriene A4 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Leukotriene A4 ELISA kit

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  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
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Description: A competitive ELISA for quantitative measurement of Human Leukotriene A4 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Leukotriene C4 ELISA kit

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Description: A competitive ELISA for quantitative measurement of Human Leukotriene C4 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Leukotriene C4 ELISA kit

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EUR 520
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
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Description: A competitive ELISA for quantitative measurement of Human Leukotriene C4 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Leukotriene C4 ELISA kit

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  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
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Description: A competitive ELISA for quantitative measurement of Human Leukotriene C4 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Leukotriene B4 ELISA kit

E01L0037-192T 192 tests
EUR 1270
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Description: A competitive ELISA for quantitative measurement of Human Leukotriene B4 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Leukotriene B4 ELISA kit

E01L0037-48 1 plate of 48 wells
EUR 520
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
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Description: A competitive ELISA for quantitative measurement of Human Leukotriene B4 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Leukotriene B4 ELISA kit

E01L0037-96 1 plate of 96 wells
EUR 685
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
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Description: A competitive ELISA for quantitative measurement of Human Leukotriene B4 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Leukotriene D4 ELISA kit

E01L0287-192T 192 tests
EUR 1270
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
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Description: A competitive ELISA for quantitative measurement of Human Leukotriene D4 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Leukotriene D4 ELISA kit

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EUR 520
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A competitive ELISA for quantitative measurement of Human Leukotriene D4 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Leukotriene D4 ELISA kit

E01L0287-96 1 plate of 96 wells
EUR 685
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A competitive ELISA for quantitative measurement of Human Leukotriene D4 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Leukotriene E4 ELISA kit

E01L0289-192T 192 tests
EUR 1270
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A competitive ELISA for quantitative measurement of Human Leukotriene E4 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Leukotriene E4 ELISA kit

E01L0289-48 1 plate of 48 wells
EUR 520
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A competitive ELISA for quantitative measurement of Human Leukotriene E4 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Leukotriene E4 ELISA kit

E01L0289-96 1 plate of 96 wells
EUR 685
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A competitive ELISA for quantitative measurement of Human Leukotriene E4 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Leukotriene B4 ELISA Kit

ELA-E0562h 96 Tests
EUR 824

Human Leukotriene C4 ELISA Kit

ELA-E0619h 96 Tests
EUR 824

Human leukotriene D4 ELISA Kit

ELA-E0935h 96 Tests
EUR 824

Human Leukotriene(LT)ELISA Kit

GA-E1970HM-48T 48T
EUR 289

Human Leukotriene(LT)ELISA Kit

GA-E1970HM-96T 96T
EUR 466

Human Leukotriene(LT)ELISA Kit

QY-E04253 96T
EUR 361

Human Leukotriene B4 (LTB4) CLIA Kit

abx197218-96tests 96 tests
EUR 825
  • Shipped within 5-12 working days.

Human Leukotriene C4 (LTC4) CLIA Kit

abx197221-96tests 96 tests
EUR 825
  • Shipped within 5-12 working days.

Human Leukotriene D4 (LTD4) CLIA Kit

abx197224-96tests 96 tests
EUR 825
  • Shipped within 5-12 working days.

Human Leukotriene E4, LTE4 ELISA Kit

ELA-E0507h 96 Tests
EUR 824

Human Leukotriene A4, LTA4 ELISA Kit

CELI-66028h 96 Tests
EUR 824

Human Leukotriene D4, LTD4 ELISA Kit

CELI-66050h 96 Tests
EUR 824

Human Leukotriene C4, LTC4 ELISA Kit

CELI-66083h 96 Tests
EUR 824

Human Leukotriene B4, LTB4 ELISA Kit

CELI-66088h 96 Tests
EUR 824

Human Leukotriene A4 Hydrolase (LTA4H) Protein

20-abx654185
  • EUR 676.00
  • EUR 286.00
  • EUR 2082.00
  • EUR 801.00
  • EUR 481.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug
  • Shipped within 5-15 working days.

 

The choice pathway of complement amplified complement deposition. Deposited C3 fragments coated the activating IgG anti-αGal, obstructing its detection and highlighting this as a possible common caveat in research of antibody density and complement deposition.
                     

 

 

The inherent capability for complement activation by the purified carbohydrate reactive IgG anti-αGal was much like that of regular human IgG. We suggest that the beforehand reported complement inhibition by IgG anti-αGal pertains to suboptimal assay configurations, in distinction to the complement activating property of the antibodies demonstrated on this paper.